Journal: International Journal of Molecular Medicine

Article Title: Overexpression of miR-375 reverses the effects of dexamethasone on the viability, migration, invasion and apoptosis of human airway epithelial cells by targeting DUSP6

doi: 10.3892/ijmm.2022.5081

Figure Lengend Snippet: Dex treatment suppressed miR-375 expression and the viability of 9HTE cells, and promoted apoptosis in a time-dependent manner. (A) Cell viability following Dex pre-treatment for different periods of time (0, 6, 12 and 24 h) followed by incubation for a further 12 or 24 h detected using Cell Counting Kit-8 assay. (B and C) Cell apoptotic rate following Dex treatment for different periods of time (0, 6, 12 and 24 h) detected using flow cytometry. (D) Relative expression levels of miR-375, miR-let-7, miR-21, miR-19 and miR-455 in cells following Dex treatment for different periods of time (0, 6, 12 and 24 h) measured using reverse transcription-quantitative PCR. U6 was employed as an internal control. All experiments were performed in triplicate and experimental data are expressed as the mean ± standard deviation. * P<0.05, ** P<0.01 and *** P<0.001 vs. Dex 0 h. Dex, dexamethasone.

Article Snippet: 9HTE cell apoptosis was detected using flow cytometry with an Annexin V-FITC/propidium iodide (PI) apoptosis kit (A211; GeneBio Systems, Inc.) as per the manufacturer's instructions.

Techniques: Expressing, Incubation, Cell Counting, Flow Cytometry, Reverse Transcription, Real-time Polymerase Chain Reaction, Control, Standard Deviation

Journal: International Journal of Molecular Medicine

Article Title: Overexpression of miR-375 reverses the effects of dexamethasone on the viability, migration, invasion and apoptosis of human airway epithelial cells by targeting DUSP6

doi: 10.3892/ijmm.2022.5081

Figure Lengend Snippet: Overexpression of miR-375 reverses the effects of Dex treatment on the expression levels of apoptosis-related proteins. (A and B) Protein/β-actin expression levels of Bcl-2, Bax and cleaved caspase-3 following Dex treatment and transfection with miR-375 mimic measured using western blot analysis. β-actin was employed as an internal control. All experiments were performed in triplicate and experimental data are expressed as the mean ± standard deviation. *** P<0.001 vs. Control; ^ P<0.05, ^^^ P<0.001 vs. Dex + MC. MC, miR-375 mimic control; M, miR-375 mimic; Dex, dexamethasone.

Article Snippet: 9HTE cell apoptosis was detected using flow cytometry with an Annexin V-FITC/propidium iodide (PI) apoptosis kit (A211; GeneBio Systems, Inc.) as per the manufacturer's instructions.

Techniques: Over Expression, Expressing, Transfection, Western Blot, Control, Standard Deviation

Journal: International Journal of Molecular Medicine

Article Title: Overexpression of miR-375 reverses the effects of dexamethasone on the viability, migration, invasion and apoptosis of human airway epithelial cells by targeting DUSP6

doi: 10.3892/ijmm.2022.5081

Figure Lengend Snippet: Overexpression of DUSP6 reversed the effects of the overexpression of miR-375 on the expression of apoptosis-related proteins in Dex-treated 9HTE cells. (A and B) Protein/β-actin expression levels of Bcl-2, Bax and cleaved caspase-3 following Dex treatment and transfection with miR-375 mimic and DUSP6 overexpression plasmid measured using western blot analysis. β-actin was employed as an internal control. All experiments were performed in triplicate and experimental data are expressed as the mean ± standard deviation. *** P<0.001 vs. control; ^ P<0.05, ^^ P<0.01 and ^^^ P<0.001 vs. Dex + MC + NC; && P<0.01 and &&& P<0.001 vs. Dex + M + NC; ## P<0.01 and ### P<0.001 vs. Dex + MC + DUSP6. NC, negative control; DUSP6, dual specificity phosphatase 6; M, miR-375 mimic; MC, miR-375 mimic control; Dex, dexamethasone.

Article Snippet: 9HTE cell apoptosis was detected using flow cytometry with an Annexin V-FITC/propidium iodide (PI) apoptosis kit (A211; GeneBio Systems, Inc.) as per the manufacturer's instructions.

Techniques: Over Expression, Expressing, Transfection, Plasmid Preparation, Western Blot, Control, Standard Deviation, Negative Control

Journal: Oncology Letters

Article Title: Cantharidin suppresses cell growth and migration, and activates autophagy in human non-small cell lung cancer cells

doi: 10.3892/ol.2018.8141

Figure Lengend Snippet: CTD induces apoptosis in A549 cells. (A) Flow cytometric analysis of A549 cell apoptosis by double staining with Annexin V-FITC and propidium iodide (PI). (B) The protein expression of Bax, Bcl-2, active caspase-3 were determined by western blotting. The protein expression of GAPDH was as used as the internal control. The protein expressions were quantified for (C) Bcl-2, (D) Bax and (E) active caspase-3. Images were analyzed by Image-Pro Plus 6.0 software. Results are presented as the mean ± standard deviation of three independent experiments. *P<0.05 was considered to indicate a statistically significantly difference vs. NC groups. Bcl-2, B-cell lymphoma-2; Bax, Bcl-associated X; CTD, cantharidin; FITC, fluorescein isothiocyanate; NC, negative control.

Article Snippet: Cell apoptosis analysis An Annexin V-Fluorescein Isothiocyanate (FITC)/propidium iodide (PI) Apoptosis Detection kit (Beijing Chemclin Biotech Co. Ltd., Beijing, China) was used to detect apoptosis according to the manufacturer's protocol.

Techniques: Double Staining, Expressing, Western Blot, Control, Software, Standard Deviation, Negative Control